خلاصه مطلب :
Proof of principle for epitope-focusedvaccine design
Vaccines prevent infectious disease largely by inducing protective neutralizing antibodies against vulnerable epitopes.Several major pathogens have resisted traditional vaccine development, although vulnerable epitopes targeted by neutralizing antibodies have been identified for several such cases. Hence, new vaccine design methods to induce epitopespecific neutralizing antibodies are needed. Here we show, with a neutralization epitope from respiratory syncytial virus,that computational protein design can generate small, thermally and conformationally stable protein scaffolds that accurately mimic the viral epitope structure and induce potent neutralizing antibodies. These scaffolds represent promisingleads for the research and development of a human respiratory syncytial virus vaccine needed to protect infants, youngchildren and the elderly. More generally, the results provide proof of principle for epitope-focused and scaffold-basedvaccine design, and encourage the evaluation and further development of these strategies for a variety of other vaccinetargets, including antigenically highly variable pathogens such as human immunodeficiency virus and influenza.